LGALS3 Knockout cell line (JIMT-1)

EN CN
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location： Home > Products > KO Cell Lines > LGALS3 Knockout cell line (JIMT-1)

EZ-editor™ Gene-editing Series Products

   CRISPR-iScreen™ Library

CRISPR-iScreen™ Library Plasmid

CRISPR-iScreen™ Library Virus

CRISPR-iScreen™ Cell Pool

   gRNA Plasmid Bank

   Cell Lines

KO Cell Lines

Wild-type Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cas9 Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Luciferase Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

EGFP Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Stem Cell Lines

   Culture Medium

Transfection Culture Medium

Cell Monoclonal Culture Medium

EZ-Stem™ Cell Culture Medium

EZ-cryo™ cell freezing medium

   Kits

Monoclone Genotype Validation Kit

Gene Knockout kit

Mouse Genotyping Kit

Lentiviral Packaging Kit

   Lentivirus

gene KO/PM cell lines
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • CRISPR-iScreen™ Library Plasmid
  • CRISPR-iScreen™ Library Virus
  • CRISPR-iScreen™ Cell Pool
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

LGALS3 Knockout cell line (JIMT-1) Order

LGALS3 Knockout cell line (JIMT-1)
Catalog#
YKO-H121
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
LGALS3 Knockout cell line (JIMT-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
LGALS3 Knockout cell line (JIMT-1)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:5
Species
Human
Gene Name
LGALS3
Gene ID
3958
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
88%DMEM+12%FBS
Accession
CVCL_E0WF(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 3 (based on transcript LGALS3-201)
Validation: PCR+Sanger Sequencing
LGALS3 Knockout cell line (JIMT-1) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:JIMT-1		 STR Info (Cell bank)
Cell Line:JIMT-1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 121314.0121314.0
D13S317 813813
D7S820 12121212
D16S539 11111111
VWA 18181818
TH01 7777
AMEL XXXX
TPOX 8888
CSF1PO 12131213
D12S391 1717
FGA 2123
D2S1338 2424
D21S11 2731.2
D18S51 1010
D8S1179 1013
D3S1358 1818
D6S1043 1111
PENTAE 719
D19S433 1213
PENTAD 912
D1S1656 1111
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the LGALS3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the JIMT-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, LGALS3 gene knockout LGALS3 Knockout cell line (JIMT-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
LGALS3 Knockout cell line (JIMT-1)
LGALS3 Knockout cell line (JIMT-1)
Catalog#
YKO-H121
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • LGALS3 Knockout cell line (Hep G2)

    LGALS3 Knockout cell line (Hep G2)
      inquiry   More details

    Organism: Human
    Gene Name: LGALS3
    Gene ID: 3958
    Size: 1*10^6
    Catalog#: YKO-HT2108
    YKO-HT2108(Hep G2)
  • LGALS3 Knockout cell line (THP-1)

    LGALS3 Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: LGALS3
    Gene ID: 3958
    Size: 1*10^6
    Catalog#: YKO-HT0279
    YKO-HT0279(THP-1)
  • Lgals3 Knockout cell line (Raw 264.7)

    Lgals3 Knockout cell line (Raw 264.7)
      inquiry   More details

    Organism: Mouse
    Gene Name: Lgals3
    Gene ID: 16854
    Size: 1*10^6
    Catalog#: YKO-MT0135
    YKO-MT0135(Raw 264.7)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • LGALS3 Knockout cell line (Hep G2)

    LGALS3 Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: LGALS3
    Gene ID: 3958
    Size: 1*10^6
    Catalog#: YKO-HT2108
      inquiry   More details
  • LGALS3 Knockout cell line (THP-1)

    LGALS3 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: LGALS3
    Gene ID: 3958
    Size: 1*10^6
    Catalog#: YKO-HT0279
      inquiry   More details
  • Lgals3 Knockout cell line (Raw 264.7)

    Lgals3 Knockout cell line (Raw 264.7)

    Organism: Mouse
    Gene Name: Lgals3
    Gene ID: 16854
    Size: 1*10^6
    Catalog#: YKO-MT0135
      inquiry   More details

WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
Contact us